Zou L, Menon R K
Department of Pediatrics, Children's Hosp. of Pittsburgh, University of Pittsburgh School of Medicine, PA 15213, USA.
Endocrinology. 1995 Nov;136(11):5236-9. doi: 10.1210/endo.136.11.7588264.
Deletional analysis by transient transfection of COS-7 cells with the murine growth hormone (GH) receptor gene promoter for the L1 transcript identified a 16-bp enhancer element located approximately 3.0 kb upstream of the major transcription start sites. The sequence of this enhancer element suggested a binding motif for the CTF/NF-1 family of transcription factors. In electromobility shift assays (EMSA) this DNA-element formed a sequence-specific complex with nuclear proteins from COS-7 cells. Competition experiments with oligonucleotide containing a consensus binding motif for CTF/NF-1 protein(s) and supershift EMSA with an anti-CTF/NF-1 antibody established that a CTF/NF-1 like protein binds to this enhancer element.
