McEwan P E, Lindop G B, Kenyon C J
Department of Pathology, Western Infirmary, Glasgow, U.K.
Endocr Res. 1995 Feb-May;21(1-2):91-102. doi: 10.3109/07435809509030424.
The control of zonation in the adrenal cortex has been studied by measuring DNA synthesis using an analogue of thymidine, bromodeoxyuridine (BrDUrd). Groups of rats were infused with BrDUrd for 10-14 days whilst being treated with: high or low sodium diets; captopril; angiotensin II; dexamethasone; an inhibitor of nitric oxide synthesis, L-NAME. DNA synthesis in the zona glomerulosa was increased by low sodium food and angiotensin and was decreased by dexamethasone, captopril L-NAME and a high sodium diet. Dexamethasone, not manipulations of the renin-angiotensin system, affected DNA synthesis in the outer zona fasciculata. The BrDUrd index in the zona intermedia was unaffected by any of the treatments and was generally lower than in adjacent zona fasciculata and zona glomerulosa cells. Cells of the zona reticularis appeared to be regulated independent of the zona fasciculata. BrDUrd uptake in nuclei of the adrenal medulla was inversely related to blood pressure. We conclude that DNA synthesis in each adrenocortical zone is independently controlled. Migration of cells within zones after proliferation is likely.
通过使用胸腺嘧啶核苷类似物溴脱氧尿苷（BrDUrd）测量DNA合成，对肾上腺皮质的区域化控制进行了研究。给几组大鼠输注BrDUrd 10 - 14天，同时给予以下处理：高钠或低钠饮食；卡托普利；血管紧张素II；地塞米松；一氧化氮合成抑制剂L - NAME。低钠食物和血管紧张素可增加球状带中的DNA合成，而地塞米松、卡托普利、L - NAME和高钠饮食则使其减少。地塞米松而非肾素 - 血管紧张素系统的操作影响束状带外层的DNA合成。中间带的BrDUrd指数不受任何处理的影响，且通常低于相邻的束状带和球状带细胞。网状带细胞似乎独立于束状带受到调节。肾上腺髓质细胞核中的BrDUrd摄取与血压呈负相关。我们得出结论，每个肾上腺皮质区的DNA合成是独立控制的。增殖后细胞在区内迁移是可能的。
