McEwan P E, Lindop G B, Kenyon C J
Department of Pathology, University of Glasgow, United Kingdom.
Am J Physiol. 1996 Jul;271(1 Pt 1):E192-8. doi: 10.1152/ajpendo.1996.271.1.E192.
Adrenal cytogenesis was investigated in response to 1) subcutaneous infusions of angiotensin (ANG) II (200 ng.kg-1.min-1); 2) high and low sodium intake; and 3) captopril treatment (10 mg.kg-1.day-1). Cell proliferation was assessed as uptake of 5-bromo-2'-deoxyuridine (BrdUrd) into nuclei. BrdUrd, infused continuously throughout 2 wk of treatment, was detected immunocytochemically. ANG II and sodium restriction caused hypertrophy of the zona glomerulosa with two- to threefold increases in BrdUrd indexes. After captopril, the glomerulosa appeared atrophied with pyknotic nuclei, but the BrdUrd index was unchanged. Zonae intermedia and fasciculata were unaffected by any treatment. Zona reticularis epithelial cells proliferated in response to ANG II and sodium restriction but were unaffected by captopril. In the medulla, captopril increased BrdUrd uptake, whereas ANG II, but not low sodium, caused a decrease. In conclusion, 1) proliferation of the glomerulosa and reticularis cells is specifically controlled by ANG II, 2) captopril may cause glomerulosa cells to die, and 3) blood pressure may control proliferation of the medulla.
1）皮下输注血管紧张素（ANG）II（200 ng·kg-1·min-1）；2）高钠和低钠摄入；3）卡托普利治疗（10 mg·kg-1·天-1）。通过检测5-溴-2'-脱氧尿苷（BrdUrd）摄入细胞核来评估细胞增殖。在整个2周的治疗过程中持续输注BrdUrd，并通过免疫细胞化学方法进行检测。ANG II和钠限制导致球状带肥大，BrdUrd指数增加两到三倍。卡托普利治疗后，球状带出现萎缩，细胞核固缩，但BrdUrd指数未改变。束状带和中间带不受任何治疗的影响。网状带上皮细胞对ANG II和钠限制有增殖反应，但不受卡托普利的影响。在髓质中，卡托普利增加了BrdUrd摄取，而ANG II（而非低钠）导致摄取减少。总之，1）球状带和网状带细胞的增殖受ANG II特异性控制；2）卡托普利可能导致球状带细胞死亡；3）血压可能控制髓质的增殖。
