Uejima Y, Fukuchi Y, Nagase T, Matsuse T, Yamaoka M, Orimo H
Pharmaceutical Discovery Research Laboratories, Teijin Institute for Biomedical Research, Tokyo, Japan.
Exp Lung Res. 1995 Jul-Aug;21(4):631-42. doi: 10.3109/01902149509031764.
Tobacco smoke is associated with pulmonary emphysema via elastase-antielastase and oxidant-antioxidant imbalance. This study addressed the tobacco smoke-induced changes in the lungs of weanling rats with vitamin E depletion. Three-week-old Wistar rats fed on vitamin E-depleted or normal diet were intermittently exposed to tobacco smoke by Hamburg II machines for 4 weeks. Tobacco smoke significantly suppressed body weight increases, particularly in the vitamin E-depleted group. In the normal diet group, tobacco smoke induced emphysematous changes with significant increases in the mean linear intercept (Lm) and the destructive index (DI), which was supported by an increase in elastase-like activity and a decrease in elastase inhibitory capacity (EIC) in bronchoalveolar lavage (BAL) fluid. Vitamin E depletion alone altered neither Lm nor DI. In tobacco-exposed animals in addition to vitamin E depletion, elastase-like activity, EIC in BAL fluid and DI were comparable to that in tobacco-exposed animals on a normal diet. However, Lm was markedly decreased with thickened epithelium and shrunk alveolar space. These results suggest that vitamin E depletion, when linked to tobacco exposure, might induce impaired lung development in the weanling rats, which is different from the emphysematous changes.
烟草烟雾通过弹性蛋白酶 - 抗弹性蛋白酶和氧化剂 - 抗氧化剂失衡与肺气肿相关。本研究探讨了维生素E缺乏的断奶大鼠肺部因烟草烟雾引起的变化。将三周龄的Wistar大鼠分为两组，分别喂食缺乏维生素E的饮食或正常饮食，通过汉堡II型机器间歇性暴露于烟草烟雾中4周。烟草烟雾显著抑制体重增加，尤其是在维生素E缺乏组中。在正常饮食组中，烟草烟雾导致肺气肿性改变，平均线性截距（Lm）和破坏指数（DI）显著增加，支气管肺泡灌洗（BAL）液中弹性蛋白酶样活性增加和弹性蛋白酶抑制能力（EIC）降低支持了这一结果。单独的维生素E缺乏既未改变Lm也未改变DI。在除维生素E缺乏外还暴露于烟草的动物中，BAL液中的弹性蛋白酶样活性、EIC和DI与正常饮食的烟草暴露动物相当。然而，Lm显著降低，伴有上皮增厚和肺泡空间缩小。这些结果表明，与烟草暴露相关的维生素E缺乏可能会导致断奶大鼠肺部发育受损，这与肺气肿性改变不同。
