Cifuentes A, Poppe H
Laboratory of Analytical Chemistry, University of Amsterdam, The Netherlands.
Electrophoresis. 1995 Apr;16(4):516-24. doi: 10.1002/elps.1150160185.
The effect of pH and ionic strength of running buffer on peptide behavior in capillary electrophoresis (CE) is studied. A system for predictions of peptide migration in CE (SPPMCE) developed in our laboratory has been tested in a wide range of pH and buffer concentrations. The SPPMCE consists of a computer program for calculating peptide pKa values, an equation which relates peptide structures to their electrophoretic mobilities and a coupled computer program for the prediction of electropherograms. More than 25 different buffers have been employed, covering a pH range of 2-11 and a concentration range of 5-100 mM. Results from experiments are compared with the theoretical predictions. Good agreement is observed, which confirms the utility of the SPPMCE and allows fast and easy optimization of peptide separations in CE, with nothing more than the amino acid sequence of the linear peptide as the input.
研究了运行缓冲液的pH值和离子强度对毛细管电泳（CE）中肽行为的影响。我们实验室开发的一种用于预测CE中肽迁移的系统（SPPMCE）已在广泛的pH值和缓冲液浓度范围内进行了测试。SPPMCE由一个用于计算肽pKa值的计算机程序、一个将肽结构与其电泳迁移率相关联的方程以及一个用于预测电泳图谱的耦合计算机程序组成。已使用了25种以上不同的缓冲液，涵盖2-11的pH范围和5-100 mM的浓度范围。将实验结果与理论预测进行了比较。观察到良好的一致性，这证实了SPPMCE的实用性，并允许在CE中快速轻松地优化肽分离，只需将线性肽的氨基酸序列作为输入即可。
