核糖体失活蛋白的tRNA差异上调作用

Differential up-regulation by tRNAs of ribosome-inactivating proteins.

作者信息

Brigotti M, Carnicelli D, Alvergna P, Pallanca A, Sperti S, Montanaro L

机构信息

Dipartimento di Patologia sperimentale dell'Università di Bologna, Italy.

出版信息

FEBS Lett. 1995 Oct 9;373(2):115-8. doi: 10.1016/0014-5793(95)01023-8.

DOI:10.1016/0014-5793(95)01023-8

PMID:7589447

Abstract

Some plant ribosome-inactivating proteins (RIPs) with RNA-N-glycosidase activity on 28S RNA require, for the inactivation of ribosomes, the presence of macromolecular cofactors present in post-ribosomal supernatants. In the case of gelonin one of the cofactors is tRNATrp lacking one or two nucleotides at the 3'-CCA end [Brigotti, M., Carnicelli, D., Alvergna, P., Pallanca, A., Lorenzetti, R., Denaro, M., Sperti, S. and Montanaro, L. (1995) Biochem. J. 310, 249-253]. In the present study it is shown that tRNAs are involved in the up-regulation of all the cofactor-requiring RIPs up to now identified (agrostin, barley RIP, PAP and tritin, besides gelonin). Polyacrylamide gel electrophoresis shows that tRNA fractions with different mobilities stimulate different RIPs. With the identification of agrostin, the cofactor-requiring RIPs (italics) add to five out of a total of thirteen investigated: barley RIP, bryodin-R, gelonin, lychnin, momordin, momorcochin-S, PAP, saporin-6, tritin [Carnicelli, D., Brigotti, M., Montanaro, L. and Sperti, S. (1992) Biochem. Biophys. Res. Commun. 182, 579-582], agrostin, luffin, trichokirin and trichosanthin (present study).

摘要

一些对28S RNA具有RNA - N - 糖苷酶活性的植物核糖体失活蛋白（RIPs），要使核糖体失活，需要核糖体后上清液中存在的大分子辅因子。就gelonin而言，其中一种辅因子是3'-CCA末端缺少一两个核苷酸的tRNATrp [Brigotti, M., Carnicelli, D., Alvergna, P., Pallanca, A., Lorenzetti, R., Denaro, M., Sperti, S.和Montanaro, L. (1995) Biochem. J. 310, 249 - 253]。本研究表明，tRNA参与了迄今为止已鉴定的所有需要辅因子的RIPs（除gelonin外，还有agrostin、大麦RIP、PAP和tritin）的上调。聚丙烯酰胺凝胶电泳显示，具有不同迁移率的tRNA组分刺激不同的RIPs。随着agrostin的鉴定，需要辅因子的RIPs（斜体）在总共13种被研究的RIPs中增加到了5种：大麦RIP、bryodin - R、gelonin、lychnin、苦瓜素、苦瓜素 - S、PAP、皂草素 - 6、tritin [Carnicelli, D., Brigotti, M., Montanaro, L.和Sperti, S. (1992) Biochem. Biophys. Res. Commun. 182, 579 - 582]，agrostin、丝瓜素、天花粉蛋白和天花粉毒蛋白（本研究）。