对上调农吉利毒素、大麦核糖体失活蛋白和PAP-S（三种植物源核糖体失活蛋白）的tRNA的鉴定。

Identification of the tRNAs which up-regulate agrostin, barley RIP and PAP-S, three ribosome-inactivating proteins of plant origin.

作者信息

Brigotti M, Keith G, Pallanca A, Carnicelli D, Alvergna P, Dirheimer G, Montanaro L, Sperti S

机构信息

Dipartimento di Patologia Sperimentale dell'Università degli Studi di Bologna, Italy.

出版信息

FEBS Lett. 1998 Jul 17;431(2):259-62. doi: 10.1016/s0014-5793(98)00769-8.

DOI:10.1016/s0014-5793(98)00769-8

PMID:9708915

Abstract

Ribosome-inactivating proteins (RIP) are RNA-N-glycosidases widely diffused in plants which depurinate ribosomal RNA at a specific universally conserved position, A4324 in rat ribosomes. A small group of RIPs (cofactor-dependent RIPs) require ATP and tRNA to reach maximal activity on isolated ribosomes. The tRNA which stimulates gelonin was identified as tRNA(Trp). The present paper reports the identification of three other tRNAs which stimulate agrostin (tRNA(Ala)), barley RIP (tRNA(Ala), tRNA(Val)) and PAP-S (tRNA(Gly)), while for tritin-S no particular stimulating tRNA emerged. The sequences of tRNA(Val) and tRNA(Gly) correspond to the already known ones (rabbit and man, respectively). The tRNA(Ala) (anticodon IGC) identifies a new isoacceptor. Only the stimulating activity of the tRNA(Ala) for agrostin approaches the specificity previously observed for the couple gelonin-tRNA(Trp).

摘要

核糖体失活蛋白（RIP）是广泛分布于植物中的RNA-N-糖苷酶，它能在一个特定的普遍保守位置使核糖体RNA脱嘌呤，在大鼠核糖体中该位置为A4324。一小部分RIP（依赖辅因子的RIP）需要ATP和tRNA才能在分离的核糖体上达到最大活性。已确定刺激白树毒素的tRNA为tRNA（Trp）。本文报道了另外三种能刺激农杆菌素（tRNA（Ala））、大麦RIP（tRNA（Ala）、tRNA（Val））和PAP-S（tRNA（Gly））的tRNA的鉴定结果，而对于小麦毒素-S则未发现有特别的刺激tRNA。tRNA（Val）和tRNA（Gly）的序列分别与已知的序列（分别来自兔子和人）相对应。tRNA（Ala）（反密码子IGC）鉴定出一种新的同功受体。只有tRNA（Ala）对农杆菌素的刺激活性接近先前观察到的白树毒素-tRNA（Trp）对的特异性。