人CHIP28水通道蛋白在酵母分泌突变体中的功能表达
Functional expression of the human CHIP28 water channel in a yeast secretory mutant.
作者信息
Laizé V, Rousselet G, Verbavatz J M, Berthonaud V, Gobin R, Roudier N, Abrami L, Ripoche P, Tacnet F
机构信息
Département de Biologie Cellulaire et Moléculaire, Service de Biologie Cellulaire, CEA-Saclay, Gif sur Yvette, France.
出版信息
FEBS Lett. 1995 Oct 16;373(3):269-74. doi: 10.1016/0014-5793(95)01060-r.
The temperature-sensitive Saccharomyces cerevisiae mutant strain NY17, deficient in the secretory pathway (sec6-4 mutation), is used for the heterologous expression of the human CHIP28 water channel. After a heat-shock, the protein is present in partially purified post-golgi secretory vesicles. Immunodetection and water transport studies, directly made on the vesicles, showed that CHIP28 is highly expressed and active in the yeast membranes.
温度敏感型酿酒酵母突变株NY17缺乏分泌途径（sec6 - 4突变），用于人CHIP28水通道的异源表达。热休克后，该蛋白存在于部分纯化的高尔基体后分泌小泡中。对小泡直接进行的免疫检测和水转运研究表明，CHIP28在酵母膜中高度表达且具有活性。
Zotero 插件