Kron M, Marquard K, Härtlein M, Price S, Leberman R
Department of Medicine, Michigan State University, East Lansing 48824, USA.
FEBS Lett. 1995 Oct 23;374(1):122-4. doi: 10.1016/0014-5793(95)01092-s.
Lymphatic filariasis is caused by infection with the filarial nematodes Brugia malayi, Brugia timori, Wuchereria bancrofti and Onchocerca volvulus which collectively infect about 200 million persons throughout the world. Protein sequence homology analysis of a major nematode antigen suggested that it was a class II aminoacyl-tRNA synthetase. The overproduction, purification and verification that the major B. malayi antigen is an asparaginyl-tRNA synthetase is described.
淋巴丝虫病由感染丝状线虫马来布鲁线虫、帝汶布鲁线虫、班氏吴策线虫和盘尾丝虫引起，全球约有2亿人感染这些线虫。对一种主要线虫抗原的蛋白质序列同源性分析表明，它是一种II类氨酰-tRNA合成酶。本文描述了马来布鲁线虫主要抗原天冬酰胺-tRNA合成酶的过量生产、纯化及验证过程。
