Hubbard M J, McHugh N J
Department of Biochemistry, University of Otaga, Dunedin, New Zealand.
FEBS Lett. 1995 Nov 6;374(3):333-7. doi: 10.1016/0014-5793(95)01135-2.
Calbindin28kDa is implicated in cytosolic calcium transport and calciprotection functions, principally as a mobile calcium buffer. Using immunoblotting, we have found that 36% of total calbindin28kDa is in the particulate fraction of rat brain. Particulate calbindin28kDa was located both within and outside organelles and required detergent for solubilisation. Equivalent observations were made for calbindin30kDa, 27% of which was insoluble. These findings indicate that a substantial proportion of calbindin does not function as a mobile calcium buffer, and perhaps instead has a calcium signalling role through target ligands in the insoluble cellular fraction.
28kDa钙结合蛋白参与胞质钙转运和钙保护功能，主要作为一种可移动的钙缓冲剂。通过免疫印迹法，我们发现大鼠脑内总28kDa钙结合蛋白的36%存在于颗粒部分。颗粒状28kDa钙结合蛋白位于细胞器内外，溶解需要去污剂。对30kDa钙结合蛋白也有类似观察结果，其中27%不溶。这些发现表明，相当一部分钙结合蛋白并非作为可移动的钙缓冲剂发挥作用，或许反而通过不溶性细胞部分中的靶配体发挥钙信号传导作用。
