Kampfenkel K, Möhlmann T, Batz O, Van Montagu M, Inzé D, Neuhaus H E
Laboratorium voor Genetica, Universiteit Gent, Belgium.
FEBS Lett. 1995 Nov 6;374(3):351-5. doi: 10.1016/0014-5793(95)01143-3.
We have isolated an Arabidopsis thaliana cDNA encoding a highly hydrophobic membrane protein of 589 amino acids which contains 12 potential transmembrane helices and shows a high degree of similarity (43.5% identity, 66.2% similarity) to the ATP/ADP translocase of the Gram-negative bacterium Rickettsia prowazekii, an obligate intracellular parasite responsible for the epidemic typhus. This rickettsial translocator resides in the cytoplasmic membrane and allows the bacterium to exploit the host cytoplasmic ATP pool. We hypothesize that the A. thaliana homolog of the R. prowazekii ATP/ADP translocase is the functional eukaryotic equivalent and resides in the plastid inner envelope membrane where it functions as an ATP importer.
我们分离出了拟南芥的一个cDNA，它编码一种由589个氨基酸组成的高度疏水的膜蛋白，该蛋白含有12个潜在的跨膜螺旋，并且与革兰氏阴性细菌普氏立克次体（一种导致流行性斑疹伤寒的专性细胞内寄生虫）的ATP/ADP转位酶具有高度相似性（43.5%的同一性，66.2%的相似性）。这种立克次体转位蛋白存在于细胞质膜中，使细菌能够利用宿主细胞质中的ATP库。我们推测，普氏立克次体ATP/ADP转位酶的拟南芥同源物是功能性的真核等同物，存在于质体内包膜中，在那里它作为ATP进口蛋白发挥作用。
