普氏立克次氏体ATP/ADP转位酶编码基因的核苷酸序列。

Nucleotide sequence of the Rickettsia prowazekii ATP/ADP translocase-encoding gene.

作者信息

Williamson L R, Plano G V, Winkler H H, Krause D C, Wood D O

机构信息

Department of Microbiology and Immunology, University of South Alabama College of Medicine, Mobile 36688.

出版信息

Gene. 1989 Aug 15;80(2):269-78. doi: 10.1016/0378-1119(89)90291-6.

DOI:10.1016/0378-1119(89)90291-6

PMID:2555259

Abstract

The Rickettsia prowazekii ATP/ADP translocase (Tlc) gene (tlc), previously cloned in Escherichia coli was localized to a 1.6-kb chromosomal fragment. Nucleotide sequence analysis of this fragment revealed an open reading frame of 1494 bp that could encode a hydrophobic protein of 497 amino acids (aa) with an Mr of 56,668. Analysis of the deduced aa sequence revealed that it contained twelve potential membrane-spanning regions. Comparisons between the deduced aa sequence of the R. prowazekii ATP/ADP Tlc and the sequences of mitochondrial (mt) Tlc revealed no detectable homologies between the rickettsial and mt sequences. The major protein synthesized in E. coli minicells containing the rickettsial gene exhibited and Mr of approx. 34,000.

摘要

曾克隆于大肠杆菌中的普氏立克次氏体ATP/ADP转位酶（Tlc）基因（tlc）定位于一个1.6 kb的染色体片段上。对该片段的核苷酸序列分析显示，有一个1494 bp的开放阅读框，可编码一个由497个氨基酸（aa）组成的疏水蛋白，其相对分子质量（Mr）为56,668。对推导的氨基酸序列分析表明，它含有12个潜在的跨膜区域。普氏立克次氏体ATP/ADP Tlc推导的氨基酸序列与线粒体（mt）Tlc的序列比较显示，立克次氏体序列与线粒体序列之间未检测到同源性。在含有立克次氏体基因的大肠杆菌微小细胞中合成的主要蛋白质的Mr约为34,000。