Nord M, Gustafsson J A, Lund J
Department of Medical Nutrition, Karolinska Institute, Stockholm, Sweden.
FEBS Lett. 1995 Nov 6;374(3):403-6. doi: 10.1016/0014-5793(95)01159-c.
To investigate interactions between the polychlorinated biphenyl-binding protein uteroglobin and phospholipids, we used a liposome-pelletting assay. PCB-BP/uteroglobin bound to liposomes made from negatively charged phospholipids (PtdSer and PtdIns) in the presence of 5 mM calcium. No binding to liposomes made from phospholipids without net charge (PtdChol and PtdEtn) was observed, nor could we detect binding in the absence of calcium or when magnesium was substituted for calcium. This suggests that PCB-BP/uteroglobin can bind to phospholipids in vivo and may have a role in the phospholipid homeostasis of the airway and/or secretory pathway of the Clara cell.
为了研究多氯联苯结合蛋白子宫珠蛋白与磷脂之间的相互作用，我们采用了脂质体沉淀测定法。在5 mM钙存在的情况下，PCB-BP/子宫珠蛋白与由带负电荷的磷脂（磷脂酰丝氨酸和磷脂酰肌醇）制成的脂质体结合。未观察到与由不带净电荷的磷脂（磷脂酰胆碱和磷脂酰乙醇胺）制成的脂质体的结合，在没有钙的情况下或用镁替代钙时，我们也检测不到结合。这表明PCB-BP/子宫珠蛋白在体内可与磷脂结合，并且可能在克拉拉细胞气道和/或分泌途径的磷脂稳态中发挥作用。
