Philipp W J, Cole S T
Unité de Génétique Moléculaire Bactérienne, Institut Pasteur, Paris, France.
FEMS Microbiol Lett. 1995 Oct 15;132(3):263-9. doi: 10.1016/0378-1097(95)00321-u.
To facilitate comparison of the genome maps of Mycobacterium tuberculosis and M. leprae, sequence data from the M. leprae sequencing project were used to design primers suitable for the amplification of short segments from conserved genes by the polymerase chain reaction. In most cases, both organisms yielded products of identical size that were then used as probes for a comparative genomic walk. The hybridization data often, but not always, revealed a similar organization of various regions of both genomes. This approach should be useful for systematic comparisons of (myco)bacterial genomes.
为便于比较结核分枝杆菌和麻风分枝杆菌的基因组图谱，利用麻风分枝杆菌测序项目的序列数据设计引物，以便通过聚合酶链反应扩增保守基因的短片段。在大多数情况下，两种菌都产生了大小相同的产物，然后将其用作比较基因组步移的探针。杂交数据常常（但并非总是）揭示了两种基因组各个区域的相似组织方式。这种方法对于（分枝）细菌基因组的系统比较应是有用的。
