Norman E, De Smet K A, Stoker N G, Ratledge C, Wheeler P R, Dale J W
Molecular Microbiology Group, School of Biological Sciences, University of Surrey, Guildford, United Kingdom.
J Bacteriol. 1994 May;176(9):2525-31. doi: 10.1128/jb.176.9.2525-2531.1994.
The causative agents of leprosy and tuberculosis, Mycobacterium leprae and Mycobacterium tuberculosis, have a lipid-rich cell envelope which contributes to virulence and antibiotic resistance. Acyl coenzyme A carboxylase, which catalyzes the first committed step of lipid biosynthesis, consists in mycobacteria of two subunits, one of which is biotinylated. Genes from M. leprae and M. tuberculosis encoding a biotinylated protein have been cloned and sequenced. Analysis of the derived protein sequences demonstrated the presence of biotin-binding sites and putative ATP-bicarbonate interactions sites, consistent with the proteins having a biotin carboxylase function as well as their being biotin carrier proteins.
麻风病和结核病的病原体，即麻风分枝杆菌和结核分枝杆菌，具有富含脂质的细胞包膜，这有助于其致病性和抗生素耐药性。催化脂质生物合成第一步的酰基辅酶A羧化酶在分枝杆菌中由两个亚基组成，其中一个亚基是生物素化的。编码生物素化蛋白的麻风分枝杆菌和结核分枝杆菌基因已被克隆和测序。对推导的蛋白质序列分析表明存在生物素结合位点和假定的ATP-碳酸氢盐相互作用位点，这与这些蛋白质具有生物素羧化酶功能以及它们作为生物素载体蛋白的情况一致。
