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[鼠伤寒沙门氏菌非可培养态产生过程受阻突变体的分离与特性研究]

[Isolation and characteristics of Salmonella typhimurium mutants with a disrupted process of generating nonculturable forms].

作者信息

Romanova Iu M, Terekhov A A, Gintsburg A G

出版信息

Genetika. 1995 Aug;31(8):1073-8.

PMID:7590216
Abstract

A laboratory model of the induction of nonculturable forms in Salmonella typhimurium has been developed. Mutants of S. typhimurium were obtained using insertion mutagenesis via the TnPhoA transposon. These mutants were impaired in the cell transition from the vegetative to the nonculturable state assayed in this model. Mutants have various phenotypes and are located in different regions of the chromosome, as shown by the data obtained using pulsed-field electrophoresis of genomic DNA.

摘要

已建立了鼠伤寒沙门氏菌中不可培养形式诱导的实验室模型。通过TnPhoA转座子插入诱变获得了鼠伤寒沙门氏菌的突变体。在该模型中检测到，这些突变体在细胞从营养状态向不可培养状态的转变过程中受到损害。如使用基因组DNA脉冲场电泳获得的数据所示，突变体具有各种表型，且位于染色体的不同区域。

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