Buisán M, Rodríguez-Peña J M, Rotger R
Departamento de Microbiología II, Facultad de Farmacia, Universidad Complutense, Madrid, Spain.
Microb Pathog. 1994 Feb;16(2):165-9. doi: 10.1006/mpat.1994.1017.
A restriction map of the 61 kb virulence plasmid of Salmonella enteritidis was constructed and compared with the Salmonella typhimurium virulence plasmid map. Polymerase chain reaction was used to detect a region homologous to rck gene in the S. enteritidis plasmid which was localized in the map by hybridization. Regions homologous to traT and vagD/vagC loci were not detected in this plasmid by this method.
构建了肠炎沙门氏菌61kb毒力质粒的限制酶切图谱,并与鼠伤寒沙门氏菌毒力质粒图谱进行了比较。采用聚合酶链反应检测肠炎沙门氏菌质粒中与rck基因同源的区域,该区域通过杂交定位在图谱上。用该方法未在该质粒中检测到与traT和vagD/vagC基因座同源的区域。