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肠炎沙门氏菌毒力质粒的限制酶切图谱及其与鼠伤寒沙门氏菌质粒的同源性。

Restriction map of the Salmonella enteritidis virulence plasmid and its homology with the plasmid of Salmonella typhimurium.

作者信息

Buisán M, Rodríguez-Peña J M, Rotger R

机构信息

Departamento de Microbiología II, Facultad de Farmacia, Universidad Complutense, Madrid, Spain.

出版信息

Microb Pathog. 1994 Feb;16(2):165-9. doi: 10.1006/mpat.1994.1017.

Abstract

A restriction map of the 61 kb virulence plasmid of Salmonella enteritidis was constructed and compared with the Salmonella typhimurium virulence plasmid map. Polymerase chain reaction was used to detect a region homologous to rck gene in the S. enteritidis plasmid which was localized in the map by hybridization. Regions homologous to traT and vagD/vagC loci were not detected in this plasmid by this method.

摘要

构建了肠炎沙门氏菌61kb毒力质粒的限制酶切图谱,并与鼠伤寒沙门氏菌毒力质粒图谱进行了比较。采用聚合酶链反应检测肠炎沙门氏菌质粒中与rck基因同源的区域,该区域通过杂交定位在图谱上。用该方法未在该质粒中检测到与traT和vagD/vagC基因座同源的区域。

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