Yebra M J, Bhagwat A S
Department of Chemistry, Wayne State University, Detroit, MI 48202, USA.
Gene. 1995 Oct 16;164(1):71-4. doi: 10.1016/0378-1119(95)00516-9.
An unusual cluster of tandemly repeated DNA sequences (TRS) was found downstream from the gene encoding DsaV methyltransferase, the DNA modification enzyme in the DsaV restriction-modification system found in a strain of Dactylococcopsis salina (Ds). The repeat unit is about 32-bp long and is present 13 times in the cluster. Each repeat unit can be divided into two distinct parts based on the level of sequence conservation and evolution. Hybridization of Ds DNA with a probe specific for this cluster revealed that there were at least two additional sites within the genome with similar TRS. The TRS units are localized in one region of the Ds genome. They do not share significant sequence similarity with other TRS found in prokaryotes.
在盐生指球藻（Ds）菌株中发现的DsaV限制修饰系统中的DNA修饰酶——DsaV甲基转移酶编码基因的下游，发现了一组不寻常的串联重复DNA序列（TRS）。重复单元约32个碱基对长，在该簇中出现13次。根据序列保守性和进化水平，每个重复单元可分为两个不同的部分。用针对该簇的特异性探针与Ds DNA杂交表明，基因组中至少还有两个具有相似TRS的位点。TRS单元位于Ds基因组的一个区域。它们与原核生物中发现的其他TRS没有显著的序列相似性。
