蓝藻基因组中的高度重复DNA序列。

Mazel D, Houmard J, Castets A M, Tandeau de Marsac N

Centre National de la Recherche Scientifique, URA D1129, Département de Biochimie et Génétique Moléculaire, Institut Pasteur, Paris, France.

J Bacteriol. 1990 May;172(5):2755-61. doi: 10.1128/jb.172.5.2755-2761.1990.

We characterized three distinct families of repeated sequences in the genome of the cyanobacterium Calothrix sp. strain PCC 7601. These repeated sequences were present at a level of about 100 copies per Calothrix genome and consisted of tandemly amplified heptanucleotides. These elements were named short tandemly repeated repetitive (STRR) sequences. We used the three different Calothrix STRR sequences as probes to perform Southern hybridization experiments with DNAs extracted from various cyanobacterial strains, Bacillus subtilis, and Escherichia coli. The three different STRR sequences were found as repetitive genomic DNA components specific to the heterocystous strains tested. The role of the STRR sequences, as well as their possible use in taxonomic studies, is discussed.

我们对蓝细菌眉藻属（Calothrix sp.）菌株PCC 7601基因组中的三种不同重复序列家族进行了表征。这些重复序列在每个眉藻基因组中的含量约为100个拷贝，由串联扩增的七核苷酸组成。这些元件被命名为短串联重复重复（STRR）序列。我们使用三种不同的眉藻STRR序列作为探针，对从各种蓝细菌菌株、枯草芽孢杆菌和大肠杆菌中提取的DNA进行Southern杂交实验。发现这三种不同的STRR序列是受试异形胞菌株特有的重复基因组DNA成分。文中讨论了STRR序列的作用及其在分类学研究中的可能用途。