A study of alkaline phosphatase isoenzyme electrophoresis on cellulose acetate compared with agar, agarose and acrylamide in the presence or absence of triton X-100.

The application of Triton X-100 in cellulose acetate electrophoresis and its effects on the separation of serum alkaline phosphatases is described. The cellulose acetate electrophoretic patterns of serum alkaline phosphatase found in liver and bone disease have been compared with the patterns found in agar, agarose, and polyacrylamide gel in the presence and absence of Triton X-100 and the differences described and discussed. Changes in the alkaline phosphatase electrophoretic pattern and the effects on certain electrophoretic variants have been noted following addition of Triton X-100 to all parts of the system.