The analysis of alkaline phosphatase isoenzyme using 4-methylumbelliferyl phosphate as substrate on a cellulose acetate membrane.
作者信息
Watanabe F, Takano M, Tanaka F, Amino N, Hayashi C, Miyai K
出版信息
Clin Chim Acta. 1979 Feb 1;91(3):273-6. doi: 10.1016/0009-8981(79)90483-2.
PMID:761403
Abstract
A new procedure is established for the analysis of alkaline phosphatase isoenzymes. The electrophoretic separation on cellulose acetate membrane coupled with the detection of alkaline phosphatase activity with 4-methyl-umbelliferyl phosphate as a substrate is described. The proposed method would be useful for the analysis of sample of micro-scale quantities and low activities.
摘要
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