Caimi G, Lo Presti R, Canino B, Montana M, Ventimiglia G, Catania A, Sarno A
Istituto di Clinica Medica e Malattie Cardiovascolari, Università di Palermo, Italy.
Horm Metab Res. 1995 Aug;27(8):352-5. doi: 10.1055/s-2007-979977.
Considering the role played by platelets and leucocytes in diabetic disease and keeping in mind the strong correlation between functional and metabolic aspects that characterizes this clinical condition, we evaluated, in two groups of diabetics, respectively the platelet and polymorphonuclear (PMN) cytosolic Ca2+ content (employing the fluorescent probe Fura 2-AM) and membrane fluidity (using the fluorescent probe TMA-DPH and considering the fluorescence polarization degree, inversely related to the membrane fluidity). From the obtained results, it is evident that the platelet cytosolic Ca2+ content does not distinguish normals from diabetics of type 1 and 2; the platelet membrane fluidity instead does not discriminate normals from diabetics, but differentiates diabetics of type 1 and 2 (type 1 = 0.284 +/- 0.015; type 2 = 0.314 +/- 0.018; p < 0.001). PMN cytosolic Ca2+ content and membrane fluidity do not discriminate normals from diabetics. In the two groups of diabetics none of the platelet and PMN parameters (cytosolic Ca2+ content and membrane fluidity) are related to the glycometabolic pattern.
考虑到血小板和白细胞在糖尿病疾病中所起的作用，并牢记表征这种临床状况的功能和代谢方面之间的强相关性，我们在两组糖尿病患者中分别评估了血小板和多形核白细胞（PMN）的胞质Ca2+含量（使用荧光探针Fura 2-AM）和膜流动性（使用荧光探针TMA-DPH并考虑与膜流动性呈反比的荧光偏振度）。从获得的结果来看，很明显血小板胞质Ca2+含量无法区分正常人与1型和2型糖尿病患者；相反，血小板膜流动性无法区分正常人与糖尿病患者，但可以区分1型和2型糖尿病患者（1型 = 0.284 +/- 0.015；2型 = 0.314 +/- 0.018；p < 0.001）。PMN胞质Ca2+含量和膜流动性无法区分正常人与糖尿病患者。在两组糖尿病患者中，血小板和PMN的参数（胞质Ca2+含量和膜流动性）均与糖代谢模式无关。
