Simon D, Carr B I
Medical College of Pennsylvania, Philadelphia 19129, USA.
Hepatology. 1995 Nov;22(5):1393-8.
We have studied the genetic profile of the host genome and hepatitis B virus (HBV) in HBV-associated primary hepatocellular carcinoma (HCC). Comparative analyses of HCC cell line Hep 40 and the original biopsy specimens showed the episomal and replicating form of HBV only in the biopsy specimen from nontumor (NT) cirrhotic liver tissue, where a molecular change in the 1p36 region was detected (NT tissue showed a normal 46XY karyotype). In contrast, only integrated HBV was detected in HCC tumor (T) tissue and Hep 40 cells. Two HBV integration sites were identical in HCC tissue and the hyperdiploid Hep 40 cell line, where genetic alteration in the 1p36 region was identified. These data indicate that viral replication is ongoing only in NT cirrhotic-hyperplastic, chromosomally normal tissue with evidence for genetic instability. Only the tumor cell with altered genotype has virus integrated
我们研究了乙肝病毒（HBV）相关原发性肝细胞癌（HCC）中宿主基因组和乙肝病毒（HBV）的基因谱。对肝癌细胞系Hep 40和原始活检标本的比较分析显示，仅在非肿瘤（NT）肝硬化肝组织的活检标本中检测到HBV的游离型和复制型，在该标本中检测到1p36区域的分子变化（NT组织显示正常的46XY核型）。相比之下，在肝癌肿瘤（T）组织和Hep 40细胞中仅检测到整合型HBV。在肝癌组织和超二倍体Hep 40细胞系中，两个HBV整合位点相同，在该细胞系中鉴定出1p36区域的基因改变。这些数据表明，病毒复制仅在NT肝硬化-增生性、染色体正常且有基因不稳定证据的组织中进行。只有基因型改变的肿瘤细胞才有病毒整合。
