克隆抗原和抗独特型抗体。
Cloned antigens and antiidiotypes.
作者信息
Herlyn D, Somasundaram R, Zaloudik J, Li W, Jacob L, Harris D, Kieny M P, Ricciardi R, Gonczol E, Sears H
机构信息
Wistar Institute, Philadelphia, PA 19104, USA.
出版信息
Hybridoma. 1995 Apr;14(2):159-66. doi: 10.1089/hyb.1995.14.159.
Both monoclonal and polyclonal antiidiotypic antibodies mimicking the human colorectal carcinoma (CRC) associated antigen CO17-1A/GA733 have induced antigen-specific humoral and cellular immunity in CRC patients. The immune responses may underlie the clinical responses observed in some of the treated patients. Recently, the CO17-1A/GA733 antigen has been molecularly cloned and expressed in baculo-, adeno-, and vaccinia viruses. In preclinical studies, these recombinant antigen preparations elicited specific humoral immunity (cytotoxic antibodies) and cellular immunity (DTH-reactive and proliferative T cells). Antibody titers elicited in animals by recombinant antigen were significantly higher than those elicited by antiidiotypes. The recombinant antigen has a potential as a vaccine for CRC patients.
模拟人类结直肠癌（CRC）相关抗原CO17 - 1A/GA733的单克隆和多克隆抗独特型抗体，均已在CRC患者中诱导出抗原特异性体液免疫和细胞免疫。这些免疫反应可能是部分接受治疗患者所观察到的临床反应的基础。最近，CO17 - 1A/GA733抗原已被分子克隆，并在杆状病毒、腺病毒和痘苗病毒中表达。在临床前研究中，这些重组抗原制剂引发了特异性体液免疫（细胞毒性抗体）和细胞免疫（迟发型超敏反应性和增殖性T细胞）。重组抗原在动物体内引发的抗体滴度显著高于抗独特型抗体所引发的滴度。该重组抗原具有作为CRC患者疫苗的潜力。
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