Zangerle R, Fuchs D, Reibnegger G, Werner-Felmayer G, Gallati H, Wachter H, Werner E R
Department of Dermatology and Venereology, University of Innsbruck, Ludwig Boltzmann Institute of AIDS Research, Austria.
Immunobiology. 1995 Jun;193(1):59-70. doi: 10.1016/S0171-2985(11)80155-5.
To get a measure of the extent of induction of nitric oxide synthase in infection with human immunodeficiency virus type-1 (HIV-1) in vivo, we estimated serum nitrite plus nitrate concentrations in 110 HIV-1 infected individuals compared to 76 blood donors. To monitor cytokine action and to measure induction of pteridine synthesis, we determined in parallel neopterin, biopterin, soluble tumor necrosis factor-alpha receptor 55 and 75, and beta 2-microglobulin. Serum nitrite plus nitrate concentrations were elevated in patients as compared to blood donor controls. In sera of patients, nitrite plus nitrate levels correlated significantly with neopterin, soluble tumor necrosis factor receptor 55 and 75, and beta 2-microglobulin. Nitrite plus nitrate levels were higher and correlations were stronger in groups of patients with lower CD4+ cell count. These results suggest that cytokine-mediated nitric oxide synthesis occurs in individuals with HIV-1 infection.
为了衡量体内感染人类免疫缺陷病毒1型（HIV-1）时一氧化氮合酶的诱导程度，我们估算了110名HIV-1感染者与76名献血者血清中亚硝酸盐加硝酸盐的浓度。为了监测细胞因子的作用并测量蝶呤合成的诱导情况，我们同时测定了新蝶呤、生物蝶呤、可溶性肿瘤坏死因子-α受体55和75以及β2-微球蛋白。与献血者对照相比，患者血清中亚硝酸盐加硝酸盐的浓度升高。在患者血清中，亚硝酸盐加硝酸盐水平与新蝶呤、可溶性肿瘤坏死因子受体55和75以及β2-微球蛋白显著相关。CD4+细胞计数较低的患者组中亚硝酸盐加硝酸盐水平更高且相关性更强。这些结果表明，HIV-1感染个体中存在细胞因子介导的一氧化氮合成。
