Asano K, Matsuo S, Okamoto K
Department of Medical Biology, School of Medicine, Showa University, Tokyo, Japan.
Immunol Lett. 1995 May;46(1-2):195-8. doi: 10.1016/0165-2478(95)00046-8.
FK-506 administered into mice daily at a dose of 10.0 mg/kg (but not 1.0 and 5.0 mg/kg) caused suppression of protective immunity to Hymenolepis nana, when the agent was injected intraperitoneally during the induction phase of protective immunity. Daily administration of 10.0 mg/kg FK-506, during the course of larval development from challenge, also suppressed protective immunity. Inhibition of protective immunity was only observed in mice that received FK-506 for 6 days at a daily dose of 10.0 mg/kg and were challenged 24 h after the final FK-506 injection. FK-506 did not inhibit formation of effector cells that mediate delayed-type hypersensitivity (DTH) to H. nana egg antigen when the agent was administered intraperitoneally at a dose of 10.0 mg/kg/day for 6 days before cell preparation. However, FK-506 did inhibit DTH effector cell activation when cells prepared from infected, saline-injected mice were transferred into 10.0 mg/kg FK-506-treated recipient mice. These results strongly indicate that FK-506 cannot inhibit the generation of effector cells but will suppress their function in vivo.
在保护性免疫诱导阶段腹腔注射FK-506时，以10.0毫克/千克（而非1.0和5.0毫克/千克）的剂量每日给小鼠注射，会抑制对微小膜壳绦虫的保护性免疫。在从攻击开始的幼虫发育过程中，每日给予10.0毫克/千克FK-506也会抑制保护性免疫。仅在以10.0毫克/千克的日剂量接受FK-506治疗6天且在最后一次FK-506注射后24小时受到攻击的小鼠中观察到保护性免疫受到抑制。当在制备细胞前6天以10.0毫克/千克/天的剂量腹腔注射FK-506时，FK-506不会抑制介导对微小膜壳绦虫卵抗原迟发型超敏反应（DTH）的效应细胞的形成。然而，当将从感染盐水注射小鼠制备的细胞转移到接受10.0毫克/千克FK-506治疗的受体小鼠中时，FK-506确实会抑制DTH效应细胞的激活。这些结果有力地表明，FK-506不会抑制效应细胞的产生，但会在体内抑制其功能。
