一种诱导交叉反应抗体的抗原肽，该抗体可抑制变形链球菌PAc与人唾液成分的相互作用。
An antigenic peptide inducing cross-reacting antibodies inhibiting the interaction of Streptococcus mutans PAc with human salivary components.
作者信息
Senpuku H, Miyauchi T, Hanada N, Nisizawa T
机构信息
Department of Oral Science, National Institute of Health, Tokyo, Japan.
出版信息
Infect Immun. 1995 Dec;63(12):4695-703. doi: 10.1128/iai.63.12.4695-4703.1995.
Abstract
A 190-kDa surface protein antigen (PAc) of Streptococcus mutans, in particular the A region of this molecule, may be implicated in the induction of dental caries via an interaction with salivary components. For this reason, it was probably used successfully as an antigenic component for experimental vaccination to prevent dental caries in animals. While developing a synthetic peptide vaccine for dental caries, as reported herein, we have identified a unique peptide, TYEAALKQYEADL, as a candidate vaccinal immunogen. The amino acid sequence of this peptide completely corresponds to the sequence of a B-cell epitope in the A region of PAc and additionally contains its own T-cell epitope for B10.D2 mice within the molecule. This peptide strongly induces the production of only cross-reacting antibodies against PAc. In addition, as demonstrated by surface plasmon resonance analysis using the BIAcore system, these cross-reacting antibodies inhibit approximately 50% of the binding of fluid-phase salivary components to immobilized recombinant PAc.
摘要
变形链球菌的一种190 kDa表面蛋白抗原（PAc），特别是该分子的A区域，可能通过与唾液成分的相互作用参与龋齿的诱导。因此，它可能成功地用作实验性疫苗接种的抗原成分，以预防动物龋齿。如本文所述，在开发用于龋齿的合成肽疫苗时，我们鉴定出一种独特的肽TYEAALKQYEADL作为候选疫苗免疫原。该肽的氨基酸序列与PAc A区域中B细胞表位的序列完全对应，并且在分子内还包含其自身针对B10.D2小鼠的T细胞表位。该肽强烈诱导仅针对PAc的交叉反应抗体的产生。此外，如使用BIAcore系统进行的表面等离子体共振分析所示，这些交叉反应抗体抑制约50%的液相唾液成分与固定化重组PAc的结合。
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