结核分枝杆菌硫氧还蛋白的鉴定及功能表征
Identification and functional characterization of thioredoxin of Mycobacterium tuberculosis.
作者信息
Wieles B, Nagai S, Wiker H G, Harboe M, Ottenhoff T H
机构信息
Immunohematology and Blood Bank, University Hospital Leiden, The Netherlands.
出版信息
Infect Immun. 1995 Dec;63(12):4946-8. doi: 10.1128/iai.63.12.4946-4948.1995.
Abstract
We have previously described a Mycobacterium tuberculosis protein designated MPT46 that was present in culture filtrates. Here we report that the MPT46 protein is thioredoxin of M. tuberculosis. MPT46 is recognized by antibodies to thioredoxin (Trx) of Escherichia coli, and antibodies of MPT46 recognize Mycobacterium leprae Trx. Moreover, MPT46 was shown to have enzymatic activity identical to that of Trx of other species, such as its ability to reduce insulin. These findings identify MPT46 as a functionally active Trx.
摘要
我们之前曾描述过一种存在于培养滤液中的结核分枝杆菌蛋白，命名为MPT46。在此我们报告，MPT46蛋白是结核分枝杆菌的硫氧还蛋白。MPT46可被抗大肠杆菌硫氧还蛋白（Trx）的抗体识别，而MPT46的抗体也能识别麻风分枝杆菌的Trx。此外，MPT46已被证明具有与其他物种Trx相同的酶活性，比如其还原胰岛素的能力。这些发现表明MPT46是一种具有功能活性的Trx。
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