Richman A D, Kao T H, Schaeffer S W, Uyenoyama M K
Department of Zoology, Duke University, Durham, NC 27708-0325, USA.
Heredity (Edinb). 1995 Oct;75 ( Pt 4):405-15. doi: 10.1038/hdy.1995.153.
S-allele diversity in Solanum carolinense was surveyed in two natural populations, located in Tennessee and North Carolina, with a molecular assay to determine the genotype of individual plants. A total of 13 different S-alleles were identified and sequenced. There is high overlap between the two populations sampled, with 10 alleles shared in common, one allele found only in Tennessee, and two found only in North Carolina. The number of alleles in this species appears to be extremely low compared with other species with gametophytic self-incompatibility. Sequence comparisons show that most alleles are extremely different one from another in their primary sequence and a phylogenetic analysis indicates extensive trans-specific evolution of S-lineages. In addition, some alleles appear to be derived much more recently. The implications of these observations are discussed in the light of recent theoretical results on S-allele population diversity and persistence.
利用分子检测方法来确定单株植物的基因型，对卡罗来纳茄两个自然种群（分别位于田纳西州和北卡罗来纳州）的S等位基因多样性进行了调查。共鉴定并测序了13种不同的S等位基因。在采样的两个种群之间存在高度重叠，共有10个等位基因相同，一个等位基因仅在田纳西州发现，两个仅在北卡罗来纳州发现。与其他具有配子体自交不亲和性的物种相比，该物种的等位基因数量似乎极低。序列比较表明，大多数等位基因在其一级序列上彼此差异极大，系统发育分析表明S谱系存在广泛的跨物种进化。此外，一些等位基因似乎是最近才衍生出来的。根据最近关于S等位基因种群多样性和持久性的理论结果，对这些观察结果的意义进行了讨论。
