Richman A D, Uyenoyama M K, Kohn J R
Department of Biology 0116, University of California at San Diego, La Jolla 92093-0116, USA.
Heredity (Edinb). 1996 May;76 ( Pt 5):497-505. doi: 10.1038/hdy.1996.72.
Allelic diversity at the self-incompatibility (S-) locus in the ground cherry, Physalis crassifolia (Solanaceae), was surveyed in a natural population occurring in Deep Canyon, CA, using a molecular assay to determine the genotype of individual plants. A total of 28 different S-alleles were identified and sequenced from a sample of 22 plants. All plants examined were heterozygous, as expected under gametophytic self-incompatibility (GSI). The estimated number of alleles in this population is 43-44, comparable to allelic diversity reported for other species, as determined by the standard diallel crossing method. Allele frequencies in the sample deviated from the expectation of equal frequency under GSI; it is suggested that this deviation may result from sampling of related individuals. Molecular analysis of genotypes within single pollen donor families indicates that, for all alleles examined, segregation is consistent with predictions for single-locus GSI. The implications of a reliable and efficient molecular assay for determining the S-genotype of plants are discussed.
利用分子分析方法来确定单株植物的基因型，对生长在加利福尼亚州深峡谷的酸浆属植物毛酸浆（茄科）自然种群中的自交不亲和（S-）位点的等位基因多样性进行了调查。从22株植物的样本中总共鉴定并测序了28个不同的S-等位基因。正如配子体自交不亲和（GSI）所预期的那样，所有检测的植物都是杂合子。该种群中等位基因的估计数量为43 - 44个，与通过标准双列杂交法确定的其他物种报道的等位基因多样性相当。样本中的等位基因频率偏离了GSI下等频率的预期；有人认为这种偏差可能是由于相关个体的采样造成的。对单个花粉供体家族内基因型的分子分析表明，对于所有检测的等位基因，分离情况与单基因座GSI的预测一致。文中讨论了一种可靠且高效的分子分析方法用于确定植物S基因型的意义。
