Selenska-Pobell S, Gigova L, Petrova N
Department of Genetics, University of Bayreuth, Germany.
J Appl Bacteriol. 1995 Oct;79(4):425-31. doi: 10.1111/j.1365-2672.1995.tb03157.x.
Strain-specific genomic patterns of Rhizobium galegae were generated by PCR using both arbitrary and repetitive (BOX, ERIC and REP) primers. The identification of the strains was achieved also by RFLP analysis. However, the PCR genomic fingerprinting has significant advantages: it is not only simpler and faster, but it is also much more discriminative because it deals with the full bacterial genome and not only with parts of it as is the case with RFLP. In addition, both kinds of PCR fingerprinting (using arbitrary or repetitive primers) generated highly specific and reproducible patterns when parallel reactions with total bacterial DNA, extracted from independent liquid cultures were performed. The latter shows that AP- and rep-PCR are convenient for controlling the production and application of Rhizobium inoculants.
利用任意引物和重复引物（BOX、ERIC和REP）通过PCR生成了特定菌株的鹰嘴豆根瘤菌基因组模式。通过RFLP分析也实现了菌株的鉴定。然而，PCR基因组指纹图谱具有显著优势：它不仅更简单、更快，而且鉴别能力更强，因为它处理的是完整的细菌基因组，而不像RFLP那样只处理其部分基因组。此外，当对从独立液体培养物中提取的总细菌DNA进行平行反应时，两种PCR指纹图谱（使用任意引物或重复引物）都产生了高度特异性和可重复的模式。后者表明，AP-PCR和rep-PCR便于控制鹰嘴豆根瘤菌接种剂的生产和应用。
