Beyer W, Mukendi F M, Kimmig P, Böhm R
Universität Hohenheim, Institut für Umwelt- und Tierhygiene sowie Tiermedizin mit Tierklinik, Stuttgart, Germany.
J Clin Microbiol. 1998 Jun;36(6):1549-54. doi: 10.1128/JCM.36.6.1549-1554.1998.
Three molecular typing methods, repetitive-sequence-based PCR (rep-PCR) fingerprinting, plasmid profiling, and arbitrarily primed PCR fingerprinting, were used to characterize isolates of Salmonella enterica serovar Saintpaul. Most of the isolates were obtained from epidemic human cases of food-borne salmonellosis, together with some from the food material suspected to be the source of infection, and a few were obtained from other cases apparently not related to the epidemic. All three methods adequately discriminated the epidemic strain from other strains of the serovar. In addition several isolates from human cases which are not identical to the epidemic strain were found. These isolates therefore must have been responsible for some sporadic infections, which were only temporally related to the epidemic. These strains showed a high degree of similarity to a strain isolated from a turkey. rep-PCR fingerprinting with REP-Dt primers and primer ERIC1R, applicable even to crude cell lysates, offers an attractive choice as a primary method for the discrimination of various Salmonella serotypes as well as isolates within serotype Saintpaul.
采用三种分子分型方法，即基于重复序列的聚合酶链反应（rep-PCR）指纹图谱法、质粒图谱分析和任意引物聚合酶链反应指纹图谱法，对肠炎沙门氏菌圣保罗血清型菌株进行特征分析。大多数菌株分离自食源性沙门氏菌病的流行期人类病例，还有一些来自疑似感染源的食品原料，少数菌株则来自其他明显与该疫情无关的病例。所有这三种方法都能充分区分流行菌株与该血清型的其他菌株。此外，还发现了几株来自人类病例但与流行菌株不同的菌株。因此，这些菌株必定导致了一些散发感染，这些感染仅在时间上与该疫情相关。这些菌株与从一只火鸡分离出的菌株高度相似。使用REP-Dt引物和ERIC1R引物进行rep-PCR指纹图谱分析，甚至适用于粗细胞裂解物，作为区分各种沙门氏菌血清型以及圣保罗血清型内菌株的主要方法，是一个有吸引力的选择。
