Nakamura M
Koga Hospital Medical Research Institute, Kurume University School of Medicine.
Nihon Rai Gakkai Zasshi. 1995 Jul;64(2):119-23. doi: 10.5025/hansen1977.64.119.
Evidence was presented in the previous paper that the activity of cells of Mycobacterium leprae was maintained in the phosphate buffer(pH 7) containing fetal calf serum (10%) with/without glycerin (2%) for approximately 4 weeks during incubation of cells at 30 degrees C, when the inoculum having more than 3,000 pg ATP was used. In the present paper, it is confirmed that the definite inoculum sizes are essential for obtaining the reproducible results that are described above, by using the inocula containing more and less than 3,000 pg ATP.
上一篇论文中提出的证据表明，当使用含有超过3000 pg ATP的接种物时，在30℃孵育细胞期间，麻风分枝杆菌细胞的活性在含有（10%）胎牛血清且有/无（2%）甘油的磷酸盐缓冲液（pH 7）中维持约4周。在本文中，通过使用含有多于和少于3000 pg ATP的接种物，证实了确定的接种量对于获得上述可重复的结果至关重要。
