Nakamura M
Kurume University, Koga Hospital Medical Research Institute, Japan.
Int J Lepr Other Mycobact Dis. 1995 Mar;63(1):28-34.
The effect of the pH of a cell-free liquid medium on the activity of Mycobacterium leprae during incubation of the cells was investigated. As a parameter for evaluating the activity, the amount of adenosine triphosphate (ATP) extracted from the incubated cells collected by centrifugation was measured. The results demonstrate that the activity of M. leprae cells was maintained at a significant level for approximately 4 weeks at 30 degrees C in 0.05 M phosphate buffer containing 10% fetal calf serum at pH 7.0 compared to cells at other pHs tested, but activity was not preserved in phosphate buffer at pH 7.0 without serum and incubated at 37 degrees C. The maintenance of the activity under these conditions was prolonged somewhat by the addition of glycerin (2%) to the medium, and was definitely inhibited by rifampin but not by either penicillin or isoniazid. From the results reported here, it could be postulated that the optimal pH of cell-free media for the study of cultivation of M. leprae is 7.0.
研究了无细胞液体培养基的pH值对麻风分枝杆菌细胞培养过程中活性的影响。作为评估活性的参数，测量了从通过离心收集的培养细胞中提取的三磷酸腺苷（ATP）的量。结果表明，与测试的其他pH值的细胞相比，麻风分枝杆菌细胞在30℃下于pH 7.0的含有10%胎牛血清的0.05 M磷酸盐缓冲液中活性可维持在显著水平约4周，但在无血清且pH 7.0的磷酸盐缓冲液中于37℃培养时活性无法保持。在这些条件下，通过向培养基中添加甘油（2%）可使活性维持时间稍有延长，且利福平可明确抑制活性，但青霉素或异烟肼则无此作用。根据此处报道的结果，可以推测用于麻风分枝杆菌培养研究的无细胞培养基的最佳pH值为7.0。
