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1
Antigenic determinants of the membrane-bound hydrogenase in Alcaligenes eutrophus are exposed toward the periplasm.嗜糖产碱菌中膜结合氢化酶的抗原决定簇朝向周质暴露。
J Bacteriol. 1995 Nov;177(21):6309-12. doi: 10.1128/jb.177.21.6309-6312.1995.
2
The membrane-bound hydrogenase of Alcaligenes eutrophus: II. Localization and immunological comparison with other hydrogenase systems.嗜糖产碱菌的膜结合氢化酶：II. 定位及与其他氢化酶系统的免疫学比较
Antonie Van Leeuwenhoek. 1980;46(1):1-14. doi: 10.1007/BF00422224.
3
Structural organization of the membrane-bound hydrogenase isolated from Alcaligenes eutrophus as revealed by electron microscopy.通过电子显微镜揭示的从嗜中性产碱杆菌分离出的膜结合氢化酶的结构组织。
FEMS Microbiol Lett. 1989 Jul 15;51(1):159-63. doi: 10.1016/0378-1097(89)90500-4.
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A gene complex coding for the membrane-bound hydrogenase of Alcaligenes eutrophus H16.编码真养产碱杆菌H16膜结合氢化酶的基因复合体。
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Comparison of the membrane-bound hydrogenases from Alcaligenes eutrophus H16 and Alcaligenes eutrophus type strain.嗜碱产碱菌H16与嗜碱产碱菌模式菌株的膜结合氢化酶比较。
Biochim Biophys Acta. 1987 Dec 11;905(2):435-46. doi: 10.1016/0005-2736(87)90473-1.
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Immunological comparison of subunits isolated from various hydrogenases of aerobic hydrogen bacteria.从需氧氢细菌的各种氢化酶中分离出的亚基的免疫学比较。
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Structural aspects and immunolocalization of the F420-reducing and non-F420-reducing hydrogenases from Methanobacterium thermoautotrophicum Marburg.嗜热自养甲烷杆菌马堡菌株中F420还原型和非F420还原型氢化酶的结构特征及免疫定位
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Molecular and immunological comparison of membrane-bound, H2-oxidizing hydrogenases of Bradyrhizobium japonicum, Alcaligenes eutrophus, Alcaligenes latus, and Azotobacter vinelandii.慢生根瘤菌、嗜糖产碱菌、产碱杆菌和棕色固氮菌的膜结合型H2氧化氢化酶的分子和免疫学比较
J Bacteriol. 1985 Jul;163(1):15-20. doi: 10.1128/jb.163.1.15-20.1985.
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Frankia KB5 possesses a hydrogenase immunologically related to membrane-bound.弗兰克氏菌KB5拥有一种与膜结合免疫相关的氢化酶。
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Energy Conservation and Hydrogenase Function in Methanogenic Archaea, in Particular the Genus .产甲烷古菌中的能量守恒与氢化酶功能，特别是. 属
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J Bacteriol. 1999 Jul;181(13):4076-80. doi: 10.1128/JB.181.13.4076-4080.1999.
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Transcriptional regulation of Alcaligenes eutrophus hydrogenase genes.嗜中温产碱菌氢化酶基因的转录调控
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Subforms and in vitro reconstitution of the NAD-reducing hydrogenase of Alcaligenes eutrophus.嗜碱假单胞菌NAD还原氢化酶的亚基形式及体外重组
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7
The Alcaligenes eutrophus membrane-bound hydrogenase gene locus encodes functions involved in maturation and electron transport coupling.嗜中温产碱杆菌膜结合氢化酶基因座编码参与成熟和电子传递偶联的功能。
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[A submersion method for culture of hydrogen-oxidizing bacteria: growth physiological studies].[一种用于氢氧化细菌培养的浸没方法：生长生理学研究]
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Structural aspects and immunolocalization of the F420-reducing and non-F420-reducing hydrogenases from Methanobacterium thermoautotrophicum Marburg.嗜热自养甲烷杆菌马堡菌株中F420还原型和非F420还原型氢化酶的结构特征及免疫定位
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Quantitative immunocytochemical localization of pancreatic secretory proteins in subcellular compartments of the rat acinar cell.大鼠腺泡细胞亚细胞区室中胰腺分泌蛋白的定量免疫细胞化学定位
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5
Depression of hydrogenase during limitation of electron donors and derepression of ribulosebisphosphate carboxylase during carbon limitation of Alcaligenes eutrophus.在真养产碱杆菌电子供体受限期间氢化酶的抑制以及碳源受限期间核酮糖二磷酸羧化酶的去抑制作用
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Nickel requirement for active hydrogenase formation in Alcaligenes eutrophus.嗜糖假单胞菌中活性氢化酶形成所需的镍
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Alcaligenes eutrophus hydrogenase genes (Hox).嗜水气单胞菌氢化酶基因（Hox）
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Enhancement of structural preservation and immunocytochemical staining in low temperature embedded pancreatic tissue.低温包埋胰腺组织中结构保存和免疫细胞化学染色的增强
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Regulation of glutamine synthetase. XII. Electron microscopy of the enzyme from Escherichia coli.谷氨酰胺合成酶的调控。十二。大肠杆菌中该酶的电子显微镜观察
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The methanoreductosome: a high-molecular-weight enzyme complex in the methanogenic bacterium strain Gö1 that contains components of the methylreductase system.甲烷还原体：产甲烷菌菌株Gö1中的一种高分子量酶复合物，其包含甲基还原酶系统的组分。
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嗜糖产碱菌中膜结合氢化酶的抗原决定簇朝向周质暴露。

Antigenic determinants of the membrane-bound hydrogenase in Alcaligenes eutrophus are exposed toward the periplasm.

作者信息

Eismann K, Mlejnek K, Zipprich D, Hoppert M, Gerberding H, Mayer F

机构信息

Institut für Mikrobiologie, Georg-August-Universität zu Göttingen, Germany.

出版信息

J Bacteriol. 1995 Nov;177(21):6309-12. doi: 10.1128/jb.177.21.6309-6312.1995.

DOI:10.1128/jb.177.21.6309-6312.1995
PMID:7592402
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC177477/
Abstract

Electron microscopic immunogold labeling experiments were performed with ultrathin sections of plasmolyzed cells of Alcaligenes eutrophus and "whole-mount" samples of spheroplasts and protoplasts. They demonstrated that antigenic determinants of the membrane-bound hydrogenase are exposed, at the outside of the cytoplasmic membrane, to the periplasm.

摘要

利用真养产碱菌质壁分离细胞的超薄切片以及原生质球和原生质体的“整装”样品进行了电子显微镜免疫金标记实验。实验表明，膜结合氢化酶的抗原决定簇在细胞质膜外侧暴露于周质空间。