Eismann K, Mlejnek K, Zipprich D, Hoppert M, Gerberding H, Mayer F
Institut für Mikrobiologie, Georg-August-Universität zu Göttingen, Germany.
J Bacteriol. 1995 Nov;177(21):6309-12. doi: 10.1128/jb.177.21.6309-6312.1995.
Electron microscopic immunogold labeling experiments were performed with ultrathin sections of plasmolyzed cells of Alcaligenes eutrophus and "whole-mount" samples of spheroplasts and protoplasts. They demonstrated that antigenic determinants of the membrane-bound hydrogenase are exposed, at the outside of the cytoplasmic membrane, to the periplasm.
利用真养产碱菌质壁分离细胞的超薄切片以及原生质球和原生质体的“整装”样品进行了电子显微镜免疫金标记实验。实验表明,膜结合氢化酶的抗原决定簇在细胞质膜外侧暴露于周质空间。