由于质粒维持导致的基因表达增强。
Gene expression enhancement due to plasmid maintenance.
作者信息
Diaz-Ricci J C, Bode J, Rhee J I, Schügerl K
机构信息
Departamento de Bioquímica de la Nutrición, Instituto Superior de Investigaciones Biológicas (CONICET-UNT), Tucumán, Argentina.
出版信息
J Bacteriol. 1995 Nov;177(22):6684-7. doi: 10.1128/jb.177.22.6684-6687.1995.
Abstract
Analysis of the chromosomic beta-galactosidase activity in strains of Escherichia coli with and without plasmids indicated that plasmid maintenance enhances gene expression. Cyclic AMP (cAMP) determinations confirmed that the gene enhancement observed in strains carrying plasmids was due to a small increase in the intracellular concentration of cAMP. Also, cells carrying plasmids displayed higher specific glucose uptake rates than did cells without plasmids. The increases in the expression of beta-galactosidase and the glucose uptake rate suggest a cAMP-mediated release of the glucose effect due to plasmid maintenance. Our results suggested that this effect is independent of the host and type and number of plasmids.
摘要
对携带和不携带质粒的大肠杆菌菌株中的染色体β-半乳糖苷酶活性进行分析表明，质粒维持可增强基因表达。环磷酸腺苷（cAMP）测定证实，在携带质粒的菌株中观察到的基因增强是由于细胞内cAMP浓度略有增加。此外，携带质粒的细胞比不携带质粒的细胞表现出更高的比葡萄糖摄取率。β-半乳糖苷酶表达和葡萄糖摄取率的增加表明，由于质粒维持，cAMP介导了葡萄糖效应的释放。我们的结果表明，这种效应与宿主、质粒的类型和数量无关。
相似文献
1
Gene expression enhancement due to plasmid maintenance.由于质粒维持导致的基因表达增强。
J Bacteriol. 1995 Nov;177(22):6684-7. doi: 10.1128/jb.177.22.6684-6687.1995.
2
Cyclic AMP inhibits and putrescine represses expression of the speA gene encoding biosynthetic arginine decarboxylase in Escherichia coli.环磷酸腺苷（cAMP）抑制，而腐胺抑制大肠杆菌中编码生物合成型精氨酸脱羧酶的speA基因的表达。
J Bacteriol. 1991 Jun;173(12):3615-21. doi: 10.1128/jb.173.12.3615-3621.1991.
3
[Study of the regulation of crp gene expression in Escherichia coli K12].[大肠杆菌K12中crp基因表达调控的研究]
Mol Gen Mikrobiol Virusol. 1990 Mar(3):18-21.
4
[Amplification of the adenylate cyclase gene in Escherichia coli cells].[大肠杆菌细胞中腺苷酸环化酶基因的扩增]
Mol Biol (Mosk). 1986 Jan-Feb;20(1):154-62.
5
Mechanism for iron control of the Vibrio fischeri luminescence system: involvement of cyclic AMP and cyclic AMP receptor protein and modulation of DNA level.费氏弧菌发光系统铁控制的机制：环磷酸腺苷和环磷酸腺苷受体蛋白的参与以及DNA水平的调节。
J Biolumin Chemilumin. 1992 Jul;7(3):203-14. doi: 10.1002/bio.1170070307.
6
Relief of catabolite repression in a cAMP-independent catabolite gene activator mutant of Escherichia coli.大肠杆菌中一个不依赖环腺苷酸（cAMP）的分解代谢基因激活突变体中分解代谢物阻遏的解除
Res Microbiol. 2004 Mar;155(2):76-9. doi: 10.1016/j.resmic.2003.11.002.
7
Effect of plasmid copy number and lac operator sequence on antibiotic-free plasmid selection by operator-repressor titration in Escherichia coli.质粒拷贝数和乳糖操纵子序列对大肠杆菌中通过操纵子-阻遏物滴定法进行无抗生素质粒筛选的影响。
J Mol Microbiol Biotechnol. 2004;7(4):197-203. doi: 10.1159/000079828.
8
Transcriptional regulation of tyrosine phenol-lyase gene mediated through TyrR and cAMP receptor protein.通过TyrR和环磷酸腺苷受体蛋白介导的酪氨酸酚裂解酶基因的转录调控。
Biosci Biotechnol Biochem. 1999 Oct;63(10):1823-7. doi: 10.1271/bbb.63.1823.
9
cAMP receptor protein-cAMP plays a crucial role in glucose-lactose diauxie by activating the major glucose transporter gene in Escherichia coli.环磷酸腺苷受体蛋白-环磷酸腺苷通过激活大肠杆菌中的主要葡萄糖转运蛋白基因，在葡萄糖-乳糖双效生长中发挥关键作用。
Proc Natl Acad Sci U S A. 1997 Nov 25;94(24):12914-9. doi: 10.1073/pnas.94.24.12914.
10
cAMP does not have an important role in carbon catabolite repression of the Escherichia coli lac operon.环磷酸腺苷（cAMP）在大肠杆菌乳糖操纵子的碳代谢物阻遏中不发挥重要作用。
Nat Rev Microbiol. 2009 Mar;7(3):250. doi: 10.1038/nrmicro1932-c3.
引用本文的文献
1
Dual-plasmid interactions stimulate the accumulation of valencene in .双质粒相互作用刺激了金合欢烯在……中的积累。
Biotechnol Notes. 2023 Dec 11;4:127-134. doi: 10.1016/j.biotno.2023.12.004. eCollection 2023.
2
Metabolic engineering of a reduced-genome strain of Escherichia coli for L-threonine production.大肠杆菌还原基因组工程菌的构建及其用于 L-苏氨酸生产
Microb Cell Fact. 2009 Jan 7;8:2. doi: 10.1186/1475-2859-8-2.
3
Effects of the presence of ColE1 plasmid DNA in Escherichia coli on the host cell metabolism.大肠杆菌中ColE1质粒DNA的存在对宿主细胞代谢的影响。
Microb Cell Fact. 2006 Nov 17;5:34. doi: 10.1186/1475-2859-5-34.
4
Effects of growth mode and pyruvate carboxylase on succinic acid production by metabolically engineered strains of Escherichia coli.生长模式和丙酮酸羧化酶对代谢工程改造的大肠杆菌菌株琥珀酸生产的影响。
Appl Environ Microbiol. 2002 Apr;68(4):1715-27. doi: 10.1128/AEM.68.4.1715-1727.2002.
本文引用的文献
1
Studies on transformation of Escherichia coli with plasmids.大肠杆菌质粒转化的研究。
J Mol Biol. 1983 Jun 5;166(4):557-80. doi: 10.1016/s0022-2836(83)80284-8.
2
Catabolite-insensitive revertants of lac promoter mutants.乳糖启动子突变体的分解代谢物不敏感回复突变体
Proc Natl Acad Sci U S A. 1970 Jul;66(3):773-9. doi: 10.1073/pnas.66.3.773.
3
A complementation analysis of the restriction and modification of DNA in Escherichia coli.大肠杆菌中DNA限制与修饰的互补分析。
J Mol Biol. 1969 May 14;41(3):459-72. doi: 10.1016/0022-2836(69)90288-5.
4
Studies of host-plasmid interactions in recombinant microorganisms.重组微生物中宿主-质粒相互作用的研究。
Ann N Y Acad Sci. 1986;469:194-211. doi: 10.1111/j.1749-6632.1986.tb26498.x.
5
High-level production of the EcoRI endonuclease under the control of the pL promoter of bacteriophage lambda.在噬菌体λ的pL启动子控制下高水平生产EcoRI核酸内切酶。
Gene. 1985;37(1-3):229-39. doi: 10.1016/0378-1119(85)90277-x.
6
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.改良的M13噬菌体克隆载体和宿主菌株：M13mp18和pUC19载体的核苷酸序列。
Gene. 1985;33(1):103-19. doi: 10.1016/0378-1119(85)90120-9.
7
Are cyclic nucleotides involved in the initiation of mitogenic activation of human lymphocytes?环核苷酸是否参与人类淋巴细胞有丝分裂原激活的起始过程？
Biochim Biophys Acta. 1985 Aug 30;846(2):216-25. doi: 10.1016/0167-4889(85)90068-0.
8
Effect of growth rate on plasmid maintenance by Escherichia coli HB101(pAT153).生长速率对大肠杆菌HB101(pAT153)质粒维持的影响。
J Gen Microbiol. 1990 Dec;136(12):2471-80. doi: 10.1099/00221287-136-12-2471.
9
Physiological aspects of growth and recombinant DNA stability in Saccharomyces cerevisiae.酿酒酵母生长及重组DNA稳定性的生理学方面
Antonie Van Leeuwenhoek. 1991 May;59(4):269-83. doi: 10.1007/BF00583680.
10
Plasmid instabilities of single and three-plasmid systems in Escherichia coli during continuous cultivation.大肠杆菌连续培养过程中单质粒和三质粒系统的质粒稳定性
J Biotechnol. 1992 Jul;24(3):235-51. doi: 10.1016/0168-1656(92)90034-7.
Zotero 插件