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The Rhizobium meliloti groELc locus is required for regulation of early nod genes by the transcription activator NodD.苜蓿根瘤菌groELc基因座是转录激活因子NodD调控早期结瘤基因所必需的。
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苜蓿根瘤菌主要σ亚基编码基因sigA的克隆与特性分析

Cloning and characterization of the sigA gene encoding the major sigma subunit of Rhizobium meliloti.

作者信息

Rushing B G, Long S R

机构信息

Howard Hughes Medical Institute, Department of Biological Sciences, Stanford University, California 94305-5020, USA.

出版信息

J Bacteriol. 1995 Dec;177(23):6952-7. doi: 10.1128/jb.177.23.6952-6957.1995.

DOI:10.1128/jb.177.23.6952-6957.1995
PMID:7592490
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC177565/
Abstract

Using PCR to create a probe based on conserved region 2 of sigma factors, we have cloned the sigA gene coding for the major sigma factor of Rhizobium meliloti. The 684-residue protein encoded by the sigA gene was expressed in vitro in coupled transcription-translation experiments with R. meliloti extracts and migrated aberrantly in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Its deduced amino acid sequence is similar to that of RpoD of Escherichia coli and is nearly identical to that of SigA of the closely related bacterium Agrobacterium tumefaciens. Through Southern analysis, we located the gene on the R. meliloti main chromosome rather than on one of the megaplasmids. The sigA locus does not appear to be part of a macromolecular synthesis operon (MMS), as in many other bacterial species, but rather lies downstream of a partial open reading frame showing similarity to the threonine dehydrogenase gene (tdh) of E. coli.

摘要

利用聚合酶链式反应（PCR）基于σ因子保守区域2创建探针，我们克隆了编码苜蓿根瘤菌主要σ因子的sigA基因。sigA基因编码的684个氨基酸残基的蛋白质，在与苜蓿根瘤菌提取物进行的体外偶联转录-翻译实验中表达，并在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳中出现异常迁移。其推导的氨基酸序列与大肠杆菌的RpoD相似，与密切相关的细菌根癌土壤杆菌的SigA几乎相同。通过Southern分析，我们将该基因定位在苜蓿根瘤菌的主染色体上，而不是在其中一个大质粒上。与许多其他细菌物种不同，sigA基因座似乎不是大分子合成操纵子（MMS）的一部分，而是位于一个与大肠杆菌苏氨酸脱氢酶基因（tdh）相似的部分开放阅读框的下游。