Metzger R, Brown D P, Grealish P, Staver M J, Versalovic J, Lupski J R, Katz L
Abbott Laboratories, Abbott Park, IL 60064.
Gene. 1994 Dec 30;151(1-2):161-6. doi: 10.1016/0378-1119(94)90649-1.
The macromolecular synthesis (MMS) operon consists of three genes: rpsU, which encodes the S21 ribosomal protein in Bacillus subtilis (Bs), rpsU is replaced by orfP23 which encodes a protein of unknown function), dnaG, encoding the DNA primase involved in the initiation of chromosome replication, and rpoD, which encodes the principal sigma subunit of RNA polymerase. The operon was cloned in three segments from Listeria monocytogenes (Lm), initially using a probe designed from a highly conserved region of RpoD. Analysis of the nucleotide sequence revealed three genes: orfP17 (whose product, P17, is homologous to Bs P23), dnaG and rpoD. The Lm DnaG resembles the primase from Escherichia coli through the first two-thirds of the sequence. C-terminal similarity was observed between DnaG from Lm and Bs. Lm RpoD is similar to Bs SigA, shares identical DNA-binding domains with SigA, and is a member of the sigma 43 subgroup of the sigma 70 family.
大分子合成（MMS）操纵子由三个基因组成：rpsU，其在枯草芽孢杆菌（Bs）中编码S21核糖体蛋白，在单核细胞增生李斯特菌（Lm）中rpsU被orfP23取代，orfP23编码一种功能未知的蛋白质；dnaG，编码参与染色体复制起始的DNA引发酶；以及rpoD，其编码RNA聚合酶的主要σ亚基。该操纵子最初使用从RpoD高度保守区域设计的探针从单核细胞增生李斯特菌中分成三个片段进行克隆。核苷酸序列分析揭示了三个基因：orfP17（其产物P17与Bs P23同源）、dnaG和rpoD。Lm DnaG在前三分之二的序列上类似于大肠杆菌的引发酶。在Lm和Bs的DnaG之间观察到C端相似性。Lm RpoD与Bs SigA相似，与SigA具有相同的DNA结合结构域，并且是σ70家族的σ43亚组的成员。
