Kimura A, Yamaguchi K, Ikeda H, Yasuda S, Matsumura S, Tsuji T
Department of Legal Medicine, Wakayama Medical College.
J Biochem. 1995 Apr;117(4):726-9. doi: 10.1093/oxfordjournals.jbchem.a124769.
The expressions of non-muscle-type (MIIA) and brain/embryonic-type (MIIB2) myosin heavy-chain isoforms in regenerating rat liver were examined. In regenerating liver after partial hepatectomy, the level of MIIA was nearly constant, while that of MIIB2 increased transiently. The level of MIIB2 was very low in normal livers, and increased gradually and then declined with a peak at the 4th day, when the regeneration was almost completed. The level of proliferating cell nuclear antigen was highest at the 2nd day. Serine dehydratase activity in the liver decreased on partial hepatectomy, began to increase at the 5th day, and reached 71% of the control at the 7th day. These results suggest that MIIB2 plays a role in reconstruction or differentiation of the regenerating tissues rather than in proliferation of hepatocytes.
研究了再生大鼠肝脏中非肌肉型（MIIA）和脑/胚胎型（MIIB2）肌球蛋白重链亚型的表达。在部分肝切除术后的再生肝脏中，MIIA水平几乎恒定，而MIIB2水平短暂升高。MIIB2在正常肝脏中的水平非常低，随着再生几乎完成，其水平逐渐升高，然后在第4天达到峰值后下降。增殖细胞核抗原水平在第2天最高。肝脏中的丝氨酸脱水酶活性在部分肝切除术后降低，在第5天开始升高，在第7天达到对照的71%。这些结果表明，MIIB2在再生组织的重建或分化中起作用，而不是在肝细胞增殖中起作用。
