Pedraza C, Matsubara S, Muramatsu T
Department of Biochemistry, Faculty of Medicine, Kagoshima University.
J Biochem. 1995 Apr;117(4):845-9. doi: 10.1093/oxfordjournals.jbchem.a124785.
Midkine is a growth/differentiation factor which is found as a product of a retinoic acid-responsive gene. The 2.3-kb upstream sequence of the human MK gene has cis acting elements which confer retinoic acid-induced expression of fused chloramphenicol acetyl-transferase (CAT) gene in F9 embryonal carcinoma cells. In the 5'-region of the human gene, a sequence resembling the DR5-type retinoic acid-responsive element (AGGTCA-related direct repeats separated by 5 nucleotides) was present in a small block of highly homologous 5'-sequences shared by the human and mouse genes. Deletion of this direct repeat reduced retinoic acid-induced CAT gene expression. The core element in the human gene differs from the consensus sequence of retinoic acid-responsive element in two nucleotides and from the retinoic acid responsive element of the mouse MK gene in one nucleotide.
中期因子是一种生长/分化因子，它是视黄酸反应基因的产物。人类中期因子（MK）基因2.3kb的上游序列含有顺式作用元件，可使氯霉素乙酰转移酶（CAT）基因在F9胚胎癌细胞中受视黄酸诱导表达。在人类基因的5'区域，在人类和小鼠基因共有的一小段高度同源的5'序列中，存在一个类似于DR5型视黄酸反应元件（由5个核苷酸分隔的AGGTCA相关直接重复序列）的序列。删除该直接重复序列会降低视黄酸诱导的CAT基因表达。人类基因中的核心元件在两个核苷酸上与视黄酸反应元件的共有序列不同，在一个核苷酸上与小鼠MK基因的视黄酸反应元件不同。
