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Binding of histone H1 to DNA is indifferent to methylation at CpG sequences.

作者信息

Campoy F J, Meehan R R, McKay S, Nixon J, Bird A

机构信息

Institute of Cell and Molecular Biology, University of Edinburgh, United Kingdom.

出版信息

J Biol Chem. 1995 Nov 3;270(44):26473-81. doi: 10.1074/jbc.270.44.26473.

DOI:10.1074/jbc.270.44.26473
PMID:7592864
Abstract

The possibility that histone H1 binds preferentially to DNA containing 5-methylcytosine in the dinucleotide CpG is appealing, as it could help to explain the repressive effects of methylation on gene activity. In this study, the affinity of purified H1 for methylated and non-methylated DNA sequences has been tested using both naked DNA and chromatin. Based on a variety of assays (bandshifts, filter-binding assays, Southwestern blots, and nuclease sensitivity assays), we conclude that H1 has no significant preference for binding to naked methylated DNA. Similarly, H1 showed the same affinities for methylated and non-methylated DNA when assembled into chromatin in a Xenopus oocyte extract. Thus potential cooperative interaction of H1 with polynucleosomal complexes is not enhanced by the presence of DNA methylation.

摘要

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DNA methylation affects nuclear organization, histone modifications, and linker histone binding but not chromatin compaction.
DNA甲基化影响核组织、组蛋白修饰和连接组蛋白结合，但不影响染色质压缩。
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