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CpG序列处的甲基化并不影响组蛋白H1与包含非洲爪蟾5S rRNA基因的核小体的结合。

Methylation at CpG sequences does not influence histone H1 binding to a nucleosome including a Xenopus borealis 5 S rRNA gene.

作者信息

Nightingale K, Wolffe A P

机构信息

Laboratory of Molecular Embryology, NICHHD, National Institutes of Health, Bethesda, Maryland 20892.

出版信息

J Biol Chem. 1995 Mar 3;270(9):4197-200. doi: 10.1074/jbc.270.9.4197.

DOI:10.1074/jbc.270.9.4197
PMID:7876175
Abstract

We demonstrate that methylation of the 12 dinucleotide CpGs within a GC-rich DNA fragment containing a Xenopus borealis 5 S rRNA gene does not influence histone H1 binding to naked or nucleosomal 5 S DNA. Thus a simple mechanism in which histone H1 selectively associates with nucleosomes containing methylated CpG cannot explain the repressive effects of methylation on gene activity.

摘要

我们证明，在含有北方爪蟾5S rRNA基因的富含GC的DNA片段内，12个二核苷酸CpG的甲基化并不影响组蛋白H1与裸露或核小体形式的5S DNA的结合。因此，组蛋白H1选择性地与含有甲基化CpG的核小体结合这一简单机制，无法解释甲基化对基因活性的抑制作用。

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1
Methylation at CpG sequences does not influence histone H1 binding to a nucleosome including a Xenopus borealis 5 S rRNA gene.CpG序列处的甲基化并不影响组蛋白H1与包含非洲爪蟾5S rRNA基因的核小体的结合。
J Biol Chem. 1995 Mar 3;270(9):4197-200. doi: 10.1074/jbc.270.9.4197.
2
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DNA methylation affects nuclear organization, histone modifications, and linker histone binding but not chromatin compaction.DNA甲基化影响核组织、组蛋白修饰和连接组蛋白结合，但不影响染色质压缩。
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