Taborelli M, Eng L, Descouts P, Ranieri J P, Bellamkonda R, Aebischer P
GAP-Biomedical, University of Geneva, Switzerland.
J Biomed Mater Res. 1995 Jun;29(6):707-14. doi: 10.1002/jbm.820290606.
We investigated the adsorption of albumin on chemically modified gold surfaces by scanning force microscopy operating both in contact and noncontact mode. The surface modification was performed with thiol-based self-assembling molecules carrying amine or methyl groups. The albumin on the aminoethanethiol-coated gold formed a uniform layer and single molecules could be distinguished. On the dodecanethiol-coated surface the protein adsorbed in aggregates or single isolated molecules depending on the incubation time. The width of the albumin molecule on both surface was similar, but the height was much lower on the amine than on the methyl surface. This was interpreted as a difference in the conformation of albumin depending on the substrate, and could explain the promotion of cell adhesion on amine-treated polymers coated with albumin.
我们通过在接触模式和非接触模式下操作的扫描力显微镜研究了白蛋白在化学修饰金表面的吸附情况。表面修饰是用带有胺基或甲基的硫醇基自组装分子进行的。在氨基乙硫醇包覆的金表面上，白蛋白形成了均匀的层，并且可以区分单个分子。在十二烷硫醇包覆的表面上，蛋白质根据孵育时间以聚集体或单个孤立分子的形式吸附。两种表面上白蛋白分子的宽度相似，但胺基表面上的高度比甲基表面上的低得多。这被解释为取决于底物的白蛋白构象差异，并且可以解释白蛋白包被的胺处理聚合物上细胞粘附的促进作用。
