Adams A M, Soames J V, Searle R F
Department of Oral Pathology, Dental School, University of Newcastle upon Tyne, UK.
J Biomed Mater Res. 1995 Aug;29(8):999-1004. doi: 10.1002/jbm.820290812.
The cytopathic effects of a range of dental restorative cements were examined by electron microscopy using an in vitro model with cultured test cells derived from human periodontal ligament. Monolayers were fixed and processed in situ after 2, 24, and 48 h exposure. Many cells showed lysis after two hours exposure consistent with immediate acute injury. Cultures subsequently recovered, reflecting different susceptibilities of cells to injury, and at later stages showed distinct patterns of cell damage in response to different restorative materials. These were related primarily to either cytoplasmic or nuclear damage and to changes resembling apoptosis.
利用体外模型，采用源自人牙周膜的培养测试细胞，通过电子显微镜检查了一系列牙科修复用粘固粉的细胞病变效应。在暴露2小时、24小时和48小时后，对单层细胞进行原位固定和处理。许多细胞在暴露两小时后出现裂解，这与即刻急性损伤一致。随后培养物恢复，这反映了细胞对损伤的不同敏感性，并且在后期阶段，针对不同的修复材料显示出不同的细胞损伤模式。这些主要与细胞质或细胞核损伤以及类似凋亡的变化有关。
