Adams A M, Soames J V, Searle R F
Department of Oral Pathology, University of Newcastle upon Tyne, UK.
Biomaterials. 1995 Sep;16(13):1031-5. doi: 10.1016/0142-9612(95)94912-5.
The cytotoxicity of a range of dental restorative cements was assessed by continuous observation of cultures with inverted microscopy and by light microscopic study of fixed preparations, using an in vitro model with cultured test cells derived from human periodontal ligament. The sequential morphological changes observed over a seven day period showed different degrees of cell loss and patterns of injury in response to different restorative materials, reflecting primarily either nuclear or cytoplasmic damage. Attempts at recovery were frequently identified as the culture period was extended and were characterized by recolonization of denuded areas of the culture well. It was concluded that differing dental cements damage cells through a variety of mechanisms and that the test cells exhibit differing degrees of susceptibility to injury. Assays based on short-term cultures may overestimate cytotoxicity by not allowing for cell recovery from reversible injury or repopulation of monolayers by proliferation of resistant cells.
通过使用源自人牙周膜的培养测试细胞的体外模型，利用倒置显微镜连续观察培养物以及对固定制剂进行光学显微镜研究，评估了一系列牙科修复用粘固粉的细胞毒性。在七天时间内观察到的一系列形态学变化显示，不同的修复材料会导致不同程度的细胞损失和损伤模式，主要反映出细胞核或细胞质的损伤。随着培养时间的延长，经常可以看到细胞有恢复的迹象，其特征是培养孔中裸露区域重新出现细胞。得出的结论是，不同的牙科粘固粉通过多种机制损伤细胞，并且测试细胞对损伤表现出不同程度的敏感性。基于短期培养的检测方法可能会因未考虑细胞从可逆性损伤中恢复或抗性细胞增殖使单层细胞重新形成而高估细胞毒性。
