Rodger F E, Fraser H M, Duncan W C, Illingworth P J
MRC Reproductive Biology Unit, Centre for Reproductive Biology, Edinburgh, UK.
Hum Reprod. 1995 Jun;10(6):1566-70. doi: 10.1093/humrep/10.6.1566.
The mechanisms of luteal regression and rescue in women are unknown but forms of programmed cell death may be involved. The proto-oncogene bcl-2 is an important inhibitor of apoptosis but has not previously been described in the human corpus luteum. Immunohistochemical localization of bcl-2 protein was investigated in human corpora lutea obtained from women undergoing surgery during endocrine monitored menstrual cycles as well as from women who had been treated with human chorionic gonadotrophin (HCG) to prolong the luteal phase. Bcl-2 was found to be localized in granulosa-lutein, theca-lutein (as identified by co-localization of P450(17)alpha-hydroxylase) and the endothelial cells around some blood vessels. Immunoblotting demonstrated the presence of a single band of approximately MW 26 kDa. There was no apparent change in either the intensity of immunostaining or the histological localization during the normal luteal phase or following treatment with human chorionic gonadotrophin. The product of the proto-oncogene bcl-2 is present in the human corpus luteum. It is unlikely that bcl-2 expression alone is responsible for prolongation of the lifespan of the corpus luteum in early pregnancy although it is possible that the action of the bcl-2 gene present is modified by changes in other members of the bcl-2 family.
女性黄体退化和挽救的机制尚不清楚，但可能涉及程序性细胞死亡的形式。原癌基因bcl-2是细胞凋亡的重要抑制剂，但此前尚未在人类黄体中被描述。我们研究了bcl-2蛋白在人类黄体中的免疫组织化学定位，这些黄体取自在内分泌监测的月经周期中接受手术的女性，以及接受人绒毛膜促性腺激素（HCG）治疗以延长黄体期的女性。发现bcl-2定位于颗粒黄体细胞、卵泡膜黄体细胞（通过P450（17）α-羟化酶的共定位确定）以及一些血管周围的内皮细胞中。免疫印迹显示存在一条约26 kDa的单条带。在正常黄体期或用人绒毛膜促性腺激素治疗后，免疫染色强度或组织学定位均无明显变化。原癌基因bcl-2的产物存在于人类黄体中。尽管bcl-2基因的作用可能会被bcl-2家族其他成员的变化所改变，但仅bcl-2表达不太可能是导致妊娠早期黄体寿命延长的原因。
