Kobayashi F, Sagawa N, Nanbu Y, Kitaoka Y, Mori T, Fujii S, Nakamura H, Masutani H, Yodoi J
Department of Obstetrics and Gynecology, Faculty of Medicine, Kyoto University, Japan.
Hum Reprod. 1995 Jun;10(6):1603-8. doi: 10.1093/humrep/10.6.1603.
Adult T-cell leukaemia-derived factor (ADF), homologous to thioredoxin, displays various biological activities, such as radical scavenging action and the reduction of protein disulphide bonds. We examined the biochemical and immunohistochemical localization of ADF in the pregnant human uterus, using two heteroantibodies to ADF, antibody C and W. Immunohistochemically, decidua and trophoblast cells were intensely stained by antibody C. The concentration of ADF-like substance in the decidua was 95.9 ng/mg protein, determined by enzyme-linked immunosorbent assay. The molecular weight of ADF-like substance in these tissues was determined by gel electrophoresis to be 13 kDa, the same as that of recombinant ADF. These findings indicate that abundant ADF is present in decidua and trophoblast cells; the localization of such a potent dithiol reducing substance may be beneficial in protecting the fertilized egg and placental trophoblasts from the cytotoxic effects of oxygen radicals.
成人T细胞白血病衍生因子（ADF）与硫氧还蛋白同源，具有多种生物学活性，如自由基清除作用和蛋白质二硫键的还原作用。我们使用两种针对ADF的异种抗体，即抗体C和抗体W，检测了ADF在妊娠人类子宫中的生化和免疫组化定位。免疫组化结果显示，蜕膜和滋养层细胞被抗体C强烈染色。通过酶联免疫吸附测定法测定，蜕膜中ADF样物质的浓度为95.9 ng/mg蛋白质。通过凝胶电泳确定，这些组织中ADF样物质的分子量为13 kDa，与重组ADF相同。这些发现表明，蜕膜和滋养层细胞中存在大量ADF；这种强效二硫醇还原物质的定位可能有助于保护受精卵和胎盘滋养层免受氧自由基的细胞毒性作用。
