Perkins A V, Di Trapani G, McKay M S, Clarke F M
Nuffield Department of Obstetrics and Gynaecology, John Radcliffe Hospital, Oxford, UK.
Placenta. 1995 Oct;16(7):635-42. doi: 10.1016/0143-4004(95)90032-2.
An immunocytochemical investigation into the expression of thioredoxin in human reproductive tissues was performed using monoclonal antibodies produced against recombinant human thioredoxin. First trimester and term human placental villi, decidua and term fetal membranes were examined for thioredoxin content and cellular localization. In first trimester tissue strong thioredoxin staining was observed in the underlying cytotrophoblast cells and in the stromal cells present in the decidua, but not in the syncytiotrophoblast surrounding the chorionic villi. In term placental villi very little thioredoxin was observed. Term fetal membranes proved to be a rich source of thioredoxin, the most intense staining was seen in the cytotrophoblast cells in the chorionic membrane, with the amnion and decidua also showing positive immunoreactivity. The potential role/s that thioredoxin may play within the placental bed is considered.
利用针对重组人硫氧还蛋白制备的单克隆抗体，对人生殖组织中硫氧还蛋白的表达进行了免疫细胞化学研究。检测了孕早期和足月人胎盘绒毛、蜕膜及足月胎膜中的硫氧还蛋白含量和细胞定位。在孕早期组织中，在其下的细胞滋养层细胞和蜕膜中的基质细胞中观察到强硫氧还蛋白染色，但在绒毛膜绒毛周围的合体滋养层中未观察到。在足月胎盘绒毛中观察到的硫氧还蛋白很少。足月胎膜被证明是硫氧还蛋白的丰富来源，在绒毛膜的细胞滋养层细胞中可见最强的染色，羊膜和蜕膜也显示出阳性免疫反应。还考虑了硫氧还蛋白在胎盘床中可能发挥的潜在作用。
