Fukuda T, Arakawa T, Shimizu Y, Ohtani K, Higuchi K, Kobayashi K
Third Department of Internal Medicine, Osaka City University Medical School, Japan.
J Clin Gastroenterol. 1995;20 Suppl 2:S5-7. doi: 10.1097/00004836-199506002-00003.
Lansoprazole, a proton pump inhibitor, has been reported to inhibit ethanol-induced injury in rats. However, the mechanism of the protective effect is not known. This study was carried out to investigate the role of prostaglandin (PG) in the protective effect of lansoprazole. Male Wistar rats were given either 30 mg/kg of lansoprazole or vehicle alone intragastrically 30 min before administration of ethanol. They were killed to measure the gross mucosal lesions in the stomach as the ulcer index. In another experiment, 5 mg/kg of indomethacin was injected 30 min before administration of lansoprazole. The effects of 16,16-dimethyl PGE2 (dmPGE2) on ethanol-induced injury over time were compared with lansoprazole. PGE2 synthesis in gastric mucosa after administration of lansoprazole was measured by radioimmunoassay. Lansoprazole reduced gastric mucosal injury by lansoprazole. DmPGE2 significantly reduced gastric mucosal injury from 5 min after exposure to ethanol. Lansoprazole did not affect PGE2 synthesis in the gastric mucosa. These results suggest that PG may not be involved in the protective effect of lansoprazole.
兰索拉唑是一种质子泵抑制剂，据报道可抑制大鼠乙醇诱导的损伤。然而，其保护作用的机制尚不清楚。本研究旨在探讨前列腺素（PG）在兰索拉唑保护作用中的作用。雄性Wistar大鼠在给予乙醇前30分钟，分别经胃内给予30mg/kg兰索拉唑或单独给予溶媒。处死大鼠后测量胃内大体黏膜损伤情况作为溃疡指数。在另一实验中，在给予兰索拉唑前30分钟注射5mg/kg吲哚美辛。将16,16-二甲基前列腺素E2（dmPGE2）对乙醇诱导损伤随时间的影响与兰索拉唑进行比较。通过放射免疫测定法测量给予兰索拉唑后胃黏膜中PGE2的合成。兰索拉唑减轻了胃黏膜损伤。DmPGE2在接触乙醇5分钟后显著减轻了胃黏膜损伤。兰索拉唑不影响胃黏膜中PGE2的合成。这些结果表明PG可能不参与兰索拉唑的保护作用。
