Klimov A I, Rocha E, Hayden F G, Shult P A, Roumillat L F, Cox N J
Influenza Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
J Infect Dis. 1995 Nov;172(5):1352-5. doi: 10.1093/infdis/172.5.1352.
Consecutive A (H3N2) influenza virus isolates from 2 influenza virus-infected immunodeficient patients treated with amantadine were examined using a novel polymerase chain reaction (PCR)-restriction analysis for resistance to this antiviral compound. The data indicate that immunodeficient patients may shed resistant viruses for prolonged periods and with different drug resistance mutations present at different times. This PCR-restriction technique allows rapid detection of amantadine- or rimantadine-resistant strains.
对2例接受金刚烷胺治疗的感染甲型（H3N2）流感病毒的免疫缺陷患者的连续病毒分离株，采用一种新型聚合酶链反应（PCR）-限制性分析方法检测其对这种抗病毒化合物的耐药性。数据表明，免疫缺陷患者可能长时间排出耐药病毒，且在不同时间出现不同的耐药突变。这种PCR-限制性技术可快速检测金刚烷胺或金刚乙胺耐药株。
